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Do BNP and CNP suppress Ang II-induced expression of TF and PAI-1 in rat aortic endothelial cells?
Do BNP and CNP suppress Ang II-induced expression of TF and PAI-1 in rat aortic endothelial cells?
BNP and CNP suppress Ang II-induced expression of procoagulant and antifibrinolytic factors (TF and PAI-1) in endothelial cells, suggesting a protective role in vascular thrombosis.
Hypothesis-generating for natriuretic peptide antithrombotic effects in endothelium; leaves open translation to in vivo or clinical thrombosis prevention.
In the present study, we demonstrate that brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) interact with angiotensin II (Ang II) in regulative blood coagulation and fibrinolysis by suppressing the expressions of both tissue factor (TF) and plasminogen activator inhibitor-1 (PAI-1) induced by Ang II. The expressions of TF and PAI-1 mRNA were analyzed by northern blotting methods, and the activities of TF on the surface of rat aortic endothelial cells (RAECs) and PAI-1 in the culture media were respectively measured by chromogenic assay. Both BNP and CNP suppressed the expressions of TF and PAI-1 mRNA induced by Ang II in a time- and concentration-dependent manner via cGMP cascade, which suppressions were accompanied by respective decrease in activities of TF and PAI-1. However, neither the expression of tissue factor pathway inhibitor (TFPI) nor tissue-type plasminogen activator (TPA) mRNA was affected by the treatment of BNP and CNP.
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A 1999 study studied this question.
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