NEDD4L knockdown reduced lipid accumulation in non-alcoholic fatty liver disease by promoting the activity of PI3K/AKT signaling pathway

Figures

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  Fig. 1. NEDD4L expression was increased in liver tissues of NAFLD mice. A According to data from GEO database (GSE109836), Sk-hep1 cells cultured in high-fat medium had much higher NEDD4L expression. B According to data from GEO database (GSE53188), mice fed a medium-fat diet had much higher NEDD4L expression. C WB indicated that NAFLD mice exhibited much higher NEDD4L protein expression in liver tissues. ** P < 0.01

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  Fig. 2. NEDD4L knockdown reduced lipid accumulation in liver tissues of NAFLD mice. A NEDD4L protein expression was reduced in liver tissues of NEDD4L knockdown mice. B NEDD4L knockdown decreased the body weight and liver weight of NAFLD mice. C and D HE staining and Oil Red O staining revealed that NEDD4L knockdown reduced lipid droplets in liver tissues of NAFLD mice. E NEDD4L knockdown reduced liver TG level of NAFLD mice. ** P < 0.01 compared with NCD group. ## P < 0.01 compared with HFD + shNC group

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  Fig. 3. NEDD4L knockdown reduced TG level in hepatocytes. A NEDD4L knockdown reduced NEDD4L expression in primary hepatocytes treated by OA. B NEDD4L knockdown decreased lipid droplets in primary hepatocytes treated by OA. C NEDD4L knockdown declined TG level in primary hepatocytes treated by OA. ** P < 0.01 compared with the control group. ## P < 0.01 compared with OA + shNC group

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  Fig. 4. NEDD4L knockdown reduced inflammatory factor levels in NAFLD tissues and cells. A WB revealed that NEDD4L knockdown reduced the expression of TNF-α, IL-1β, and p-p65/p65 proteins in liver tissues of NAFLD mice. B NEDD4L knockdown reduced the expression of TNF-α, IL-1β, IL6 and p-p65/p65 proteins in primary hepatocytes treated by OA. ** P < 0.01 relative to NCD group or the control group. ## P < 0.01 relative to HFD + shNC group or OA + shNC group

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  Fig. 5. NEDD4L knockdown promoted the PI3K/AKT signaling pathway activity in NAFLD tissues and cells. A NEDD4L knockdown increased the phosphorylation of PI3K/AKT in liver tissues of NAFLD mice. B NEDD4L knockdown elevated the phosphorylation of PI3K/AKT in primary hepatocytes treated by OA. ** P < 0.01 relative to NCD group or the control group. ## P < 0.01 relative to HFD + shNC group or OA + shNC group