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In the present study, plant extract and CuO nanoparticles were synthesized by simple and green method by using Chrysanthemum indicum flower. Plant extract were investigate for their secondary metabolites and antioxidant activity and CuO NPs were investigate for their UV, FTIR and anticancer activity. Flower sample of Chrysanthemum indicum were collected, air- dired, pulverized and subjected to extraction with water as a solvent. The total phenol, flavonoids and tannin content, and antioxidant activity of the aqueous extract were evaluated using the DPPH, hydroxyl radicles, nitric oxide and SOD assay. The CuO NPs were characterization for UV, FTIR and cytotoxic potential activity, respectively. The total phenol, flavonoids and tannin content of the plant extract were found to be insignificant amount of bioactive compounds present. The DPPH, hydroxyl radicles, Nitric oxide assay and SOD revealed that the Chrysanthemum indicum extract exhibited strong antioxidant assay. The UV and FTIR characterization studies demonstrated the formation of synthesis CuO NPs plant extract. The cytotoxicity study reveals that plants are nontoxic to PC cell line. Study concluded that leaves of Chrysanthemum indicum were the rich source of antioxidant and phytochemicals. Based on preliminary investigation, further research should be focused to isolate bioactive phytochemicals as leading source of clinical medicine in future.
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Farshid et al. (2024) studied this question.
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