What question did this study set out to answer?

To identify the structural, chemical, and charge characteristics of molecular species that regulate fluid-phase vesicle formation in mouse macrophages.

February 1, 1967The Journal of Experimental MedicineOpen Access

The Regulation of Pinocytosis in Mouse Macrophages

View Full Paper

Ask AI

Bookmark

Authors

ZCZanvil A. CohnRockefeller Foundation EPEileen E. ParksUniversity of Oklahoma Health Sciences Center

Discussion

Member takes

Implication

In vitro study reveals that negatively charged molecules stimulate vesicle formation in mouse macrophages, indicating surface charge dictates cellular fluid uptake.

Key Points

To identify the structural, chemical, and charge characteristics of molecular species that regulate fluid-phase vesicle formation in mouse macrophages.
Exposed mouse macrophage cell cultures to diverse proteins, amino acids, polysaccharides, nucleic acids, and small anions in basal medium for 150 minutes.
Quantified cellular pinosome induction by counting vesicles per cell and comparing them against untreated controls in basal medium.
Acidic proteins (albumin and fetuin with isoelectric points of 5 or below), polyglutamic acid, and polyanions (heparin, chondroitin sulfate, DNA, and RNA) markedly stimulated vesicle formation, whereas neutral and basic macromolecules were largely ineffective.
The stimulatory capacity of albumin required bound fatty acids (restored by oleic or linoleic acid), and fetuin required intact sialic acid residues, losing activity following neuraminidase treatment or acid hydrolysis.
Unnatural D isomers of aspartic acid, glutamic acid, leucine, and phenylalanine inhibited vesicle induction, with D-glutamic acid inhibition overcome by co-treatment with the L isomer.

Cite This Study

Cohn et al. (1967) studied this question.

synapsesocial.com/papers/6a8169de1c90fa4c6da8ed0d https://doi.org/10.1084/jem.125.2.213

View Full Paper

Ask AI

Bookmark

Implication

In vitro study reveals that negatively charged molecules stimulate vesicle formation in mouse macrophages, indicating surface charge dictates cellular fluid uptake.

The Regulation of Pinocytosis in Mouse Macrophages

Discussion

Member takes

Implication

Key Points

Cite This Study

The Regulation of Pinocytosis in Mouse Macrophages

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider