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August 16, 2026Applied and Environmental MicrobiologyOpen Access

Constitutive, endogenous, fluorescent membrane reporters for dynamic cell cycle analysis in Bacillus subtilis

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Authors

JJJane JonchaSRSadie B. RuesewaldKAKehinde O Adebiyi

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Overview

Experimental study demonstrates that a fluorescent PtsG membrane reporter reliably tracks cytokinesis in Bacillus subtilis, highlighting a robust tool for dynamic cell-cycle imaging.

Key Points

  • Identify a bright, constitutive, and growth-neutral endogenous fluorescent membrane reporter to dynamically track cell division and cytokinesis in Bacillus subtilis.
  • Screened for bright, uniform membrane fusions using forward genetic transposon mutagenesis paired with fluorescence-activated cell sorting (FACS).
  • Characterized PtsGΩmsfGFP using microfluidic growth channels, fluorescence loss in photobleaching (FLIP) microscopy, and dual-color time-lapse imaging with FtsZ fusions.
  • Identified PtsGΩmsfGFP as a bright, uniform, and growth-neutral membrane reporter that functions across vegetative growth and sporulation.
  • Redefined the completion of cytokinesis as the point when membrane fluorescence reaches a local maximum at the division plane, which coincided with Z-ring recycling and mid-constriction peak FtsZ intensity.

Cite This Study

Joncha et al. (2026) studied this question.

synapsesocial.com/papers/6a8178fcf2fb91fc834ac2e7https://doi.org/10.1128/aem.01126-26
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