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July 25, 2025

A versatile dual-color bacterial reporter system highlights two distinct Pseudomonas aeruginosa Type 3 secretion system intracellular populations.

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Authors

CCChristopher J. CorcoranDGDavid G. GlanvilleZRZachary J. Resko

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Overview

Utility of a dual-color reporter system enhances protein and transcriptional tracking in bacteria, suggesting improved visualization techniques.

Key Points

  • The dual-color reporter system enables precise tracking of bacterial intracellular populations.
  • It separates populations with different gene expression states, particularly T3SS-associated exoS expression.
  • Using flow cytometry and biofilm models, protein localization and bacterial viability are effectively monitored.
  • The system allows for innovative spatiotemporal imaging in live animal models, advancing microbiological techniques.

Cite This Study

Corcoran et al. (2025) studied this question.

synapsesocial.com/papers/689a0627e6551bb0af8ce28chttps://doi.org/10.1101/2025.07.23.666013
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