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December 10, 2025ACS Synthetic BiologyOpen Access

A Reporter System for Assessment of Transcription from Divergently Oriented Promoters in Pseudomonas putida

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Authors

JHJohanna HendriksonMKMia-Lota KeskkülaGRGea M. Räis

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Overview

A novel plasmid-based reporter system measures gene expression in biofilms of Pseudomonas putida, suggesting new insights into its regulation.

Key Points

  • The study aims to develop a dual-reporter system for monitoring gene expression in Pseudomonas putida.
  • Developed a plasmid-based dual-reporter system optimized for Pseudomonas putida.
  • Used fluorescent proteins SYFP2 and Scarlet-I3 to monitor gene expression.
  • Engineered plasmids with BBR1 and RK2 origins of replication, and included a toxin-antitoxin module.
  • Assessed transcriptional activity using confocal microscopy to observe expression patterns.
  • Tested excludon-based transcriptional repression in Pseudomonas putida.
  • Successfully created a system for simultaneous monitoring of divergently oriented promoters.
  • Demonstrated distinct expression patterns of biofilm-related genes within mature biofilms.
  • Observed limited efficiency of transcriptional repression under tested conditions.

Cite This Study

Hendrikson et al. (2025) studied this question.

synapsesocial.com/papers/69401b312d562116f28f7c35https://doi.org/10.1021/acssynbio.5c00723
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