Chemical synthesis study demonstrates a convergent route to bifunctional glycosylphosphatidylinositol probes, suggesting a universal platform to analyze protein-membrane orientation.
A bifunctional glycosylphosphatidylinositol (GPI) anchor containing an azide and a biotin to facilitate additional functionalization via click chemistry and avidin conjugation, respectively, was synthesized by a convergent strategy using an orthogonal protection tactic. It is a general platform to rapidly access diverse GPI and GPI-anchored protein (GPI-AP) probes. Spin-labeled GPI and GPI-AP derived from this platform were used in a related study to show that GPI governs the membrane orientation of GPI-AP, validating GPI as a unique protein anchor.
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Yan et al. (2026) studied this question.
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