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August 16, 2026Genome

Filling DNA barcode gaps in Central European Auchenorrhyncha: a new reference library and Oxford Nanopore Technologies-based species identification

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Authors

SSSandra SchallhartJGJacqueline GrimmWHWerner E. Holzinger

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Overview

Methodological evaluation demonstrates improved species identification in Auchenorrhyncha using expanded DNA barcodes and nanopore sequencing, highlighting enhanced tools for insect biodiversity...

Key Points

  • To expand the DNA barcode reference library for Central European Auchenorrhyncha and evaluate high-throughput Oxford Nanopore Technologies sequencing for field biodiversity monitoring.
  • Integrated morphological identification with Sanger-sequenced cytochrome c oxidase subunit I (COI) barcodes across 1,304 specimens representing 394 species.
  • Implemented a high-throughput Oxford Nanopore Technologies (ONT) workflow on Malaise trap catches from lowland agricultural and alpine sites, benchmarking results against suction sampling.
  • Generated 384 Barcode Index Numbers (BINs), including 73 newly established BINs, while identifying cryptic species complexes via intraspecific divergence and BIN discordance.
  • The expanded reference database substantially increased assignment success and taxonomic resolution for ONT-sequenced bulk samples, detecting species proportions comparable to morphology-based suction sampling.

Cite This Study

Schallhart et al. (2026) studied this question.

synapsesocial.com/papers/6a8179eff2fb91fc834ad57fhttps://doi.org/10.1139/gen-2026-0009
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