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August 16, 2026InsectsOpen Access

BmMBNL Knockout Disrupts Molting of Silkworm, Bombyx mori, Through Inhibiting Expressions of Chitin Synthetase and Chitinase

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Authors

HDHuan DongZMZihan MengMHMengyao He

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Overview

Gene knockout study reveals that BmMBNL loss disrupts molting and reduces chitin in silkworms, indicating that microRNA regulation is essential for insect development.

Key Points

  • To determine the function of the microRNA-processing protein BmMBNL in regulating insect molting and chitin dynamics in Bombyx mori.
  • Characterized spatiotemporal expression profiles of BmMBNL across silkworm tissues and developmental stages.
  • Generated targeted BmMBNL knockout silkworms using CRISPR/Cas9 genome editing.
  • Analyzed larval phenotypes, chitin layer thickness, and microRNA and gene expression levels via RT-PCR and RNA-sequencing transcriptome analysis.
  • BmMBNL displayed high expression in the epidermis and was substantially upregulated during larval molting phases.
  • CRISPR/Cas9-mediated knockout of BmMBNL triggered molting failure at the 3rd and 4th instar larval stages, reduced body size, thinned the chitin layer, and caused lethality.
  • Knockout of BmMBNL upregulated miR-1, miR-71, miR-263, miR-8-5p, and miR-2a-3p, which directly suppressed chitinase expression.

Cite This Study

Dong et al. (2026) studied this question.

synapsesocial.com/papers/6a817a4cf2fb91fc834ae035https://doi.org/10.3390/insects17080849
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