What question did this study set out to answer?

To evaluate the challenges of DNA barcoding for aquatic macroinvertebrate identification and assess its utility in evaluating freshwater biological water quality.

August 16, 2026BMC ZoologyOpen Access

Assessment of aquatic macroinvertebrate diversity and water quality in Kwan Phayao, Thailand: integrating morphology and DNA barcoding

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Authors

DPDutrudi PanpromminUniversity of Phayao WPWanlaya PanyarosUniversity of Phayao JJJaruwan JaidetUniversity of Phayao

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Implication

Environmental assessment reveals moderate water quality and taxonomic diversity using DNA barcoding in freshwater macroinvertebrates, highlighting limitations in current reference databases.

Key Points

To evaluate the challenges of DNA barcoding for aquatic macroinvertebrate identification and assess its utility in evaluating freshwater biological water quality.
Analyzed N=203 macroinvertebrate specimens using morphological keys and cytochrome c oxidase subunit I (COI) gene sequencing.
Integrated phylogenetic analysis with Automatic Barcode Gap Discovery (ABGD) and evaluated water quality via physicochemical parameters alongside BMWP Thai and ASPT Thai biotic indices.
Morphological identification classified samples into 13 orders, 7 classes, and 3 phyla, whereas COI sequences and ABGD resolved 86 distinct taxa across 37 genera and 29 families.
Only 25.62% of samples were confidently identified to the species level due to reference database limitations, while biotic indices indicated moderate water quality.

Cite This Study

Panprommin et al. (2026) studied this question.

synapsesocial.com/papers/6a817a60f2fb91fc834ae191 https://doi.org/10.1186/s40850-026-00282-2

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Implication

Environmental assessment reveals moderate water quality and taxonomic diversity using DNA barcoding in freshwater macroinvertebrates, highlighting limitations in current reference databases.

Assessment of aquatic macroinvertebrate diversity and water quality in Kwan Phayao, Thailand: integrating morphology and DNA barcoding

Discussion

Member takes

Implication

Key Points

Cite This Study

Assessment of aquatic macroinvertebrate diversity and water quality in Kwan Phayao, Thailand: integrating morphology and DNA barcoding

Discussion

Member takes

Implication

Key Points

Cite This Study

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