What question did this study set out to answer?

To isolate infectious Bagaza virus from naturally infected wildlife and assess its replication kinetics, host cell tropism, and antiviral susceptibility.

August 16, 2026Scientific ReportsOpen Access

Recovery and characterization of a field derived Bagaza virus with broad cellular host range

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Authors

LALaura Albentosa-González JQJoão Queirós CGCatarina Gonçalves

Discussion

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Overview

In vitro study demonstrates broad replication of a field-isolated Bagaza virus across avian, mosquito, and human cells, highlighting potential cross-species transmission risks.

Key Points

To isolate infectious Bagaza virus from naturally infected wildlife and assess its replication kinetics, host cell tropism, and antiviral susceptibility.
Extracted viral RNA from the brain tissue of a deceased red-legged partridge and recovered infectious Bagaza virus through RNA transfection.
Evaluated viral replication kinetics across a panel of avian, mosquito, and mammalian cell lines, including monkey, hamster, duck, chicken, and human lines (HEK-293T and Huh7.5).
Visualized cytoplasmic replication complexes via immunocytochemical detection of double-stranded RNA and tested viral susceptibility to the broad-spectrum antiviral ribavirin.
Bagaza virus replicated efficiently in mosquito, duck, monkey, and specific human cell lines (HEK-293T and Huh7.5), but showed restricted replication in chicken, hamster, and other human cell models.
Double-stranded RNA staining confirmed the localization of viral RNA replication complexes within the cytoplasm of infected cells.
Viral replication was sensitive to inhibition by the antiviral nucleoside analogue ribavirin.

Cite This Study

Albentosa-González et al. (2026) studied this question.

synapsesocial.com/papers/6a817a91f2fb91fc834ae792 https://doi.org/10.1038/s41598-026-67237-y

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August 16, 2026Scientific ReportsOpen Access

Recovery and characterization of a field derived Bagaza virus with broad cellular host range

View Full Paper

Ask AI

Bookmark

Authors

LALaura Albentosa-González JQJoão Queirós CGCatarina Gonçalves

Discussion

Member takes

Overview

In vitro study demonstrates broad replication of a field-isolated Bagaza virus across avian, mosquito, and human cells, highlighting potential cross-species transmission risks.

Key Points

To isolate infectious Bagaza virus from naturally infected wildlife and assess its replication kinetics, host cell tropism, and antiviral susceptibility.
Extracted viral RNA from the brain tissue of a deceased red-legged partridge and recovered infectious Bagaza virus through RNA transfection.
Evaluated viral replication kinetics across a panel of avian, mosquito, and mammalian cell lines, including monkey, hamster, duck, chicken, and human lines (HEK-293T and Huh7.5).
Visualized cytoplasmic replication complexes via immunocytochemical detection of double-stranded RNA and tested viral susceptibility to the broad-spectrum antiviral ribavirin.
Bagaza virus replicated efficiently in mosquito, duck, monkey, and specific human cell lines (HEK-293T and Huh7.5), but showed restricted replication in chicken, hamster, and other human cell models.
Double-stranded RNA staining confirmed the localization of viral RNA replication complexes within the cytoplasm of infected cells.
Viral replication was sensitive to inhibition by the antiviral nucleoside analogue ribavirin.

Cite This Study

Albentosa-González et al. (2026) studied this question.

synapsesocial.com/papers/6a817a91f2fb91fc834ae792 https://doi.org/10.1038/s41598-026-67237-y

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