What question did this study set out to answer?

Investigate the cleavage kinetics of EcoRI restriction endonuclease at individual recognition sites on bacteriophage lambda DNA across varying reaction conditions.

November 1, 1980Biochemical JournalOpen Access

The EcoRI restriction endonuclease with bacteriophage λ DNA. Kinetic studies

View Full Paper

Ask AI

Bookmark

Authors

SHStephen E. HalfordUniversity of Bristol NJNeil P. JohnsonCreighton University JGJ. GrinstedThe Pirbright Institute

Discussion

Member takes

Implication

Biochemical study reveals site-specific cleavage rates of EcoRI on bacteriophage lambda DNA, indicating independent reaction sites influenced by flanking sequences.

Key Points

Investigate the cleavage kinetics of EcoRI restriction endonuclease at individual recognition sites on bacteriophage lambda DNA across varying reaction conditions.
Measured kinetic cleavage rates of EcoRI restriction endonuclease across individual recognition sites on bacteriophage lambda DNA under varied pH and ionic strength conditions.
Assessed whether cleavage across multiple recognition sites on a single DNA molecule operates via an independent or processive reaction mechanism.
Cleavage rates were identical across recognition sites under specific pH and ionic conditions, but diverged markedly among sites when reaction conditions were altered.
Reactions at individual recognition sites proceeded independently, ruling out a processive mechanism for the EcoRI enzyme.

Cite This Study

Halford et al. (1980) studied this question.

synapsesocial.com/papers/6a81915a6494b7aed7bf613a https://doi.org/10.1042/bj1910581

View Full Paper

Ask AI

Bookmark

Implication

Biochemical study reveals site-specific cleavage rates of EcoRI on bacteriophage lambda DNA, indicating independent reaction sites influenced by flanking sequences.

The EcoRI restriction endonuclease with bacteriophage λ DNA. Kinetic studies

Discussion

Member takes

Implication

Key Points

Cite This Study

The EcoRI restriction endonuclease with bacteriophage λ DNA. Kinetic studies

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider