A novel chlorinated coumarin-malononitrile fluorescent probe with three potential reaction sites, which exhibited highly selective, rapid response, low detection limit, and was capable of the simultaneous detection of H2S (λex/em = 515/564 nm) and GSH (λex/em = 430/517 nm), was first proposed. The probe was successfully applied to dual-channel imaging H2S and GSH in MCF-7 cells.
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Li et al. (2016) studied this question.
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