Current developments in SELDI affinity technology

I. Introduction 34 II. Affinity Probes 35 A. Initial Surface‐Affinity Research 36 B. Self‐Assembled Monolayers 36 C. Dextran 36 D. Metal Affinity 36 E. Antibody‐ and Protein‐Affinity Probes 37 F. Lectin Affinity Capture 38 G. Inherent Hydrophobic Interaction Bio‐Probes 38 H. Bio‐Molecular Interaction Probes 39 III. SELDI 39 A. Fundamentals of SELDI 39 B. SELDI in Proteomics 40 IV. Conclusions 41 References 41 The overall history and recent advancements in Surface‐Enhanced Laser Desorption/Ionization (SELDI) affinity technology is reviewed. A detailed account of SELDI technology, utilizing Immobilized‐Metal Affinity surfaces, pseudo‐specific chromatographic surfaces, and biospecific interactive surfaces, is presented with particular emphasis placed upon examination of fundamental characteristics as well as specific applications for each. Finally, a detailed review of the specific use of such affinity surfaces in fundamental aspects of clinical, process, and research proteomics activity is presented.