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The tides are turning: Kalata B1 (KB1) is the prototypical member of an exceptionally stable and resistant family of plant-derived miniproteins called cyclotides. The biosynthesis of KB1 and a small library of KB1-based cyclotides (variation occurs in five solvent-exposed loops) in E. coli has been performed by using recombinant-DNA expression techniques and protein splicing.
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Kimura et al. (2006) studied this question.
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