Key result
Plasma untargeted metabolomics identified 21 significantly altered metabolites, primarily related to phospholipid metabolism, in patients with pulmonary arterial hypertension compared to healthy controls.
Why the study?
Pulmonary arterial hypertension has a complex diagnostic schedule, and no specific indicator of its arterial etiology has been explored.
Case-Control (n=79)
No
Untargeted plasma metabolomics identified a 21-metabolite phospholipid signature that differentiates pulmonary arterial hypertension patients from controls, offering potential for diagnostic biomarker panels.
Hypothesis-generating for a phospholipid metabolomic signature in PAH; leaves open prospective validation before any diagnostic use.
Pulmonary arterial hypertension is a rare but life-threatening and clinically heterogeneous disease. The diagnostic schedule of this disorder is complex, and no specific indicator of the arterial etiology has been explored. In this study, untargeted plasma metabolomics was applied to evaluate the metabolic fingerprints of pulmonary arterial hypertension patients. Plasma samples were prepared using a new approach, which applies proteinase K during the sample preparation procedure to increase the metabolite coverage. The metabolic fingerprints were determined via LC-MS and subsequently analyzed with the use of both uni- and multivariate statistics. A total of 21 metabolites were discovered to be significantly altered in pulmonary arterial hypertensive patients. The metabolites were mainly related to the phospholipid metabolic pathways. In this study, decreases were found in the phosphatidylcholines (PCs) [PC(32:0), PC(40:7), PC(42:7)], phosphatidylethanolamine PE(18:0/18:2), lysophosphatidylethanolamines (LPEs) [LPE(22:6), LPE(18:2), LPE(18:0), LPE(20:4), LPE(20:1), LPE(20:0)], lysophosphatidylcholine LPC(20:4) and lysophosphatidylserine LPS(19:0), as well as increase of sphingomyelin SM(36:2), in the plasma samples of pulmonary arterial hypertensive patients in comparison to the control group. Besides their function as components of the biological membranes, these metabolites are also involved in the intracellular signaling pathways that are related to cell proliferation and apoptosis. The results obtained during this study confirm the potential of (untargeted) metabolomics to identify the molecular characteristics of the pathophysiology of pulmonary arterial hypertension. The clinical relevance of this study constitutes the selection of a metabolic panel that can potentially detect and properly diagnose the disease.
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Wawrzyniak et al. (2023) conducted a case-control in Pulmonary arterial hypertension (n=79). Pulmonary arterial hypertension vs. Healthy matched volunteers was evaluated on Identification of significantly altered plasma metabolites. Plasma untargeted metabolomics identified 21 significantly altered metabolites, primarily related to phospholipid metabolism, in patients with pulmonary arterial hypertension compared to healthy controls.
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