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Methodology for the detection of delta 9-tetrahydrocannabJnol (THC) In blood Is reported. THC is extracted from blood with chloroform, and extracts are purified using paper chromatography. Identification of delta 9-tetrahydrocannabJnol, cannabJdiol, and cannabJnol Is achieved by the combined use of computerized gas chromatography-mass spectrometry. The mass spectrometer is operated In the multiple ion monitoring mode, monitoring ions of m/e 231,238, 243, 271,295,299,310, and 314. The procedure was developed for forensic purposes. The detection limit of THC is 0.5 ng/mL of blood. Data of 142 cases are presented.
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Pirl et al. (1979) studied this question.
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