What question did this study set out to answer?

To resolve the covalent structure and genetic biogenesis of the capsular polysaccharide in the enterotoxin-producing, non-O1 Vibrio cholerae strain NRT36S.

March 15, 2007BMC MicrobiologyOpen Access

The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region

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To resolve the covalent structure and genetic biogenesis of the capsular polysaccharide in the enterotoxin-producing, non-O1 Vibrio cholerae strain NRT36S.
Determined the chemical structure of the capsular polysaccharide using high-resolution nuclear magnetic resonance (NMR) spectroscopy.
Identified capsule biogenesis genes using transposon mutagenesis paired with whole-genome sequencing (GenBank DQ915177).
Resolved the capsular polysaccharide structure as a complex repeating subunit composed of four residues.
Identified that the capsule biogenesis gene cluster shares the identical genetic locus occupied by the lipopolysaccharide (LPS) O-antigen biosynthesis machinery.
Demonstrated that physical co-localization of capsule and LPS genes enables the simultaneous emergence of novel surface antigens in a single bacterial strain.

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Implication

Molecular analysis reveals capsule biosynthesis genes co-localize with lipopolysaccharide loci in non-O1 Vibrio cholerae, indicating a mechanism for coordinated antigenic variation and immune evasion.

Cite This Study

Chen et al. (2007) studied this question.

synapsesocial.com/papers/6a81c15eb5fee548463c7c3e https://doi.org/10.1186/1471-2180-7-20

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The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region

Key Points

Discussion

Implication

Cite This Study

The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region

Key Points

Discussion

Implication

Cite This Study

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