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The 12α-hydroxylation of the steroid nucleus in cholic acid biosynthesis was studied in the microsomal fraction of rat liver homogenate. The reaction was shown to require NADPH. A participation of flavins in the 12α-hydroxylase system or a requirement for metal ions could not be shown. Carbon monoxide inhibited partly the 12α-hydroxylase system. The effect of some inhibitors of steroid hydroxylations was tested and the influence of sex and age was studied. The relative rates of 12α-hydroxylations of a number of C27 steroids were measured and were found to be: 7α-hydroxycholest-4-en-3-one, 1.0; 5β-cholestane-3α, 7α-dicl, 0.4; 7α-hydroxy-5β-cholestan-3-one, 0.2; cholest-5-ene-3β, 7α-diol, 0.1; cholesterol and cholest-4-en-3-one, < 0.1.
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Kurt Einarsson (1968) studied this question.
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