Rapid Energy Transfer in Cascade-Type Bodipy Dyes

This is the authors' abstract. We don't add key points for this paper.

Three new molecular dyads, comprising a bora-3a,4a-diaza-s-indacene (Bodipy) dye linked to two aromatic polycycles via the boron center, have been synthesized and fully characterized. The polycyclic compounds are either pyrene or perylene, or a mixture of both. Whereas the absorption spectral profiles contain important contributions from each of the subunits, fluorescence occurs exclusively from the Bodipy fragment. Intramolecular excitation energy transfer is extremely efficient in each case, even though spectral overlap integrals for the pyrene-based system are modest. Although these polycycles are sterically congested, molecular dynamics simulations indicate that they are in dynamic motion, and this hinders proper computation of the orientation factors for Förster-type energy transfer. These new dyes, especially the mixed polycycle system, greatly extend the range of excitation wavelengths that can be used for fluorescence microscopy.