Delta-9-Tetrahydrocannabinol alleviates doxorubicin-induced weight loss but does not affect dextran sodium sulphate-induced colitis

Figures

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  Fig. 1. Changes in mouse body weight over time in THC-treated mucositis and colitis. a Weight change (%) as compared with baseline weight in Doxorubicin (DOXO)-induced mucositis. b Weight change (%) as compared with baseline DSS-induced colitis. Veh = vehicle treatment, THC10 = 10 mg/kg THC, THC20 = 20 mg/kg THC. Results are presented as means ± SEM. Weight is relative to baseline weight on day −1. ***p ≤ 0.001, ****p ≤ 0.0001

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  Fig. 2. Changes in mouse intestinal length in THC-treated mucositis and colitis. a Small intestinal length on day 7 after start of DOXO-treatment. b Length of the colon on day 7 after start of DOXO-treatment. c Colon-caecum intestinal length at day 10 in the DSS study. BW = Body weight, Veh = vehicle treatment, THC10 = 10 mg/kg THC, THC20 = 20 mg/kg THC. Results are expressed as means ± SEM. The intestinal lengths are shown relative to baseline weight. *p < 0.05, **p ≤ 0.01

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  Fig. 3. Mucositis and histological damage scores. a Mucositis score at day 2 in the doxorubicin (DOXO)-experiment. b Mucositis score at day 7 in the DOXO-experiment. c Histological damage score in the DSS-experiment. Veh = vehicle treatment, THC10 = 10 mg/kg THC, THC20 = 20 mg/kg THC. Results are presented as means ± SEM. *p < 0.05, ***p ≤ 0.001, ****p ≤ 0.0001

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  Fig. 4. Effects of THC on expression of pro-inflammatory cytokines determined by qRT-PCR in doxorubicin-treated animals. (a, b) jejunal expression of TNF-alpha, (c, d) jejunal expression of IL-1 beta, (e, f) jejunal expression of IL-6 at day 2 and day 7 respectively. Note the difference in the y-axis scale among the cytokines. Veh = vehicle treatment, THC10 = 10 mg/kg THC, THC20 = 20 mg/kg THC. Data are shown as relative gene expression in the mean fold change ± SEM. *p < 0.05

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  Fig. 5. Effects of THC on expression of pro-inflammatory cytokines determined by qRT-PCR in DSS-treated animals. a-c represents the data from IL-1 beta, TNF-alpha and IL-6 respectively. Note the difference in the y-axis scale among the cytokines. Veh = vehicle treatment, THC10 = 10 mg/kg THC, THC20 = 20 mg/kg THC. Data are shown as relative gene expression in the mean fold change ± SEM. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001