Association of p34cdc with Replicating DNA

Two major events in the cell cycle are DNA replication and mitosis. The functionally equivalent genes in yeast, cdc2+ (Schizosaccharomyces pombe) and CDC28 (Saccharomyces cerevisiae), play a central role in cell cycle control (Hereford and Hartwell 1974; Nurse et al. 1976; Beach et al. 1982; Booher and Beach 1986). They are first required for passage through a point in G1 defined as START and again later in the cell cycle for mitosis (Nurse and Bisset 1981; Pigott et al. 1982; Booher and Beach 1987). The existence of a human homolog of cdc2 implies that this gene may perform a similar function in higher organisms (Lee and Nurse 1987). The product of cdc2 and its homolog in other organisms (p34cdc2) are known to have protein kinase activity (Simanis and Nurse 1986). Although the significance of p34cdc2 kinase in mitosis is well understood, little is known about its role in S phase...