What question did this study set out to answer?

To characterize the biochemical properties of herpes simplex virus-specified deoxypyrimidine kinase and examine whether its thymidine and deoxycytidine phosphorylating activities originate from the same catalytic site.

September 1, 1974Journal of General Virology

Biochemical Studies on the Herpes Simplex Virus-specified Deoxypyrimidine Kinase Activity

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Authors

AJA. T. JamiesonUniversity of Glasgow JSJ. H. Subak‐SharpeMRC University of Glasgow Centre for Virus Research

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Implication

Biochemical analysis reveals distinct dual-substrate phosphorylation by herpes simplex virus deoxypyrimidine kinase, suggesting both nucleosides share a single active site.

Key Points

To characterize the biochemical properties of herpes simplex virus-specified deoxypyrimidine kinase and examine whether its thymidine and deoxycytidine phosphorylating activities originate from the same catalytic site.
Compared enzymatic responses to diverse biochemical stimuli between viral deoxypyrimidine kinase and host mammalian thymidine and deoxycytidine kinases.
Attempted physical fractionation and separation of thymidine and deoxycytidine phosphorylating activities using sedimentation, electrophoretic mobility, and molecular size analysis.
HSV-specified deoxypyrimidine kinase demonstrated stimulus responses that differed significantly from host mammalian thymidine kinase and deoxycytidine kinase.
Thymidine and deoxycytidine phosphorylating activities showed distinct biochemical behaviors but could not be resolved or separated by sedimentation, electrophoresis, or size profiling.
Qualitative findings indicate that both deoxypyrimidine nucleosides are phosphorylated at the same catalytic active site.

Cite This Study

Jamieson et al. (1974) studied this question.

synapsesocial.com/papers/6a81f53e6494b7aed7bf7b7f https://doi.org/10.1099/0022-1317-24-3-481

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Implication

Biochemical analysis reveals distinct dual-substrate phosphorylation by herpes simplex virus deoxypyrimidine kinase, suggesting both nucleosides share a single active site.

Biochemical Studies on the Herpes Simplex Virus-specified Deoxypyrimidine Kinase Activity

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Implication

Key Points

Cite This Study

Biochemical Studies on the Herpes Simplex Virus-specified Deoxypyrimidine Kinase Activity

Discussion

Member takes

Implication

Key Points

Cite This Study

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