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Rapid detection of pathogenic and spoilage microorganisms is essential for ensuring the safety and quality of food. Real‐time polymerase chain reaction (PCR) technology has the potential to achieve rapid, sensitive, and specific detection of these microorganisms in food. In this review, we discuss real‐time PCR technologies in use today, applications of real‐time PCR in food systems, and some of the associated challenges and limitations.
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Hanna et al. (2005) studied this question.
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