What question did this study set out to answer?

To resolve the intersubunit structural organization of human complement component C8 and clarify how it coordinates membrane attack complex pore assembly.

November 11, 2010Journal of Molecular BiologyOpen Access

Structure of Human Complement C8, a Precursor to Membrane Attack

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Authors

DBDoryen BubeckImperial College London PRPietro RoversiUniversity of Milan RDRossen DonevThe Honourable Society of Lincoln's Inn

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Implication

Structural analysis reveals the intersubunit architecture of human complement C8, highlighting how accessible functional domains initiate membrane attack complex pore formation.

Key Points

To resolve the intersubunit structural organization of human complement component C8 and clarify how it coordinates membrane attack complex pore assembly.
Determined the overall quaternary structure of the complement C8 heterotrimer using electron microscopy.
Fitted the co-crystal structure of C8α-MACPF-C8γ and a homology model of C8β-MACPF into the reconstructed electron microscopy density map.
Demonstrated that the C8γ protrusion remains structurally accessible within the assembled C8 heterotrimer.
Identified that the membrane-inserting region of the C8α-MACPF domain is exposed prior to activation, preparing the complex for membrane penetration.

Cite This Study

Bubeck et al. (2010) studied this question.

synapsesocial.com/papers/6a821751004fcf503374a75e https://doi.org/10.1016/j.jmb.2010.10.031

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Implication

Structural analysis reveals the intersubunit architecture of human complement C8, highlighting how accessible functional domains initiate membrane attack complex pore formation.

Structure of Human Complement C8, a Precursor to Membrane Attack

Discussion

Member takes

Implication

Key Points

Cite This Study

Structure of Human Complement C8, a Precursor to Membrane Attack

Discussion

Member takes

Implication

Key Points

Cite This Study

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