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After glow. Reactive chemical adaptors were used to stabilize bioluminogenic phosphates for monitoring alkaline phosphatase (AP) activity (see scheme). The 1,6-elimination based luciferin phosphate exhibited ultrasensitive ability to detect ∼10−22 mol of AP enzyme in a homogeneous solution, and picograms of protein in an immunoassay.
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Zhou et al. (2008) studied this question.
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