What question did this study set out to answer?

To evaluate a dual-fluorescent staining method using SYBR-14 and propidium iodide for identifying and quantifying living, moribund, and dead mammalian sperm.

August 1, 1995Biology of Reproduction

Viability Assessment of Mammalian Sperm Using SYBR-14 and Propidium Iodide1

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To evaluate a dual-fluorescent staining method using SYBR-14 and propidium iodide for identifying and quantifying living, moribund, and dead mammalian sperm.
Stained sperm from six mammalian species (bulls, boars, rams, rabbits, mice, and men) with SYBR-14 and propidium iodide (PI).
Observed staining patterns and motility via fluorescence microscopy to track membrane integrity and dye localization.
Quantified the proportion of viable, moribund, and nonviable sperm using flow cytometry.
SYBR-14 selectively stained the DNA of motile, living sperm bright green, whereas propidium iodide stained nonmotile cells with damaged membranes red.
Flow cytometric analysis consistently delineated three distinct cell populations across all six mammalian species: living (SYBR-14 green), moribund (doubly stained), and dead (PI red).
Dying sperm exhibited a visible transition from green to red fluorescence that initiated at the posterior region of the sperm head and advanced anteriorly.

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Implication

Laboratory study demonstrates accurate quantification of sperm viability across six mammalian species using SYBR-14 and propidium iodide, suggesting a reliable flow cytometric assessment tool.

Cite This Study

Garner et al. (1995) studied this question.

synapsesocial.com/papers/6a8262e01b8b6a4492b4b384 https://doi.org/10.1095/biolreprod53.2.276

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Implication

Laboratory study demonstrates accurate quantification of sperm viability across six mammalian species using SYBR-14 and propidium iodide, suggesting a reliable flow cytometric assessment tool.

Viability Assessment of Mammalian Sperm Using SYBR-14 and Propidium Iodide1

Key Points

Discussion

Implication

Cite This Study

Viability Assessment of Mammalian Sperm Using SYBR-14 and Propidium Iodide1

Key Points

Discussion

Implication

Cite This Study

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