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February 1, 1994Molecular MicrobiologyOpen Access

The receptor for Bacillus thuringiensis CrylA(c) delta‐endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase N

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Authors

PKPeter J. KnightUniversity of LeedsNCNeil CrickmoreUniversity of SussexDEDavid J. EllarGoogle (United States)

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Implication

Biochemical analysis reveals that aminopeptidase N serves as the CryIA(c) delta-endotoxin receptor in Manduca sexta, highlighting the molecular target for Bacillus thuringiensis insecticidal proteins.

Key Points

  • Identify and characterize the specific midgut brush border membrane receptor for the Bacillus thuringiensis CryIA(c) delta-endotoxin in Manduca sexta larvae.
  • Purified a 120 kDa midgut membrane glycoprotein using a combination of protoxin affinity chromatography and anion exchange chromatography.
  • Evaluated binding specificity via immunoblotting against CryIA(c), CryIB, and lectin SBA in the presence of GlcNAc or GalNAc, combined with N-terminal/internal sequencing and enzymatic assays.
  • The purified 120 kDa glycoprotein bound CryIA(c) in the presence of GlcNAc but was inhibited by GalNAc, bound lectin SBA, and exhibited no binding to CryIB.
  • Peptide sequence analysis revealed high similarity to aminopeptidase N (EC 3.4.11.2), with purified receptor fractions displaying a 5.3-fold enrichment in aminopeptidase activity relative to brush border membrane vesicles.

Cite This Study

Knight et al. (1994) studied this question.

synapsesocial.com/papers/6a82635534d909b0e726fcddhttps://doi.org/10.1111/j.1365-2958.1994.tb00324.x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins1990 · 359 citations
  2. 2Signal sequences1989 · 427 citations
  3. 3An analysis of Bacillus thuringiensisδ‐endotoxin action on insect‐midgut‐membrane permeability using a light‐scattering assay1993 · 110 citations
  4. 4Anchoring and Biosynthesis of Stalked Brush Border Membrane Proteins: Glycosidases and Peptidases of Enterocytes and Renal Tubuli1986 · 412 citations
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