Answers are grounded in this paper and cite their sources.
Comprehensive two‐dimensional reversed‐phase HPLC–capillary electrophoresis (RP HPLC–CE) was used to compare the tryptic digest fingerprints of horse heart cytochrome C and bovine heart cytochrome C. The peptide fragments were labeled with fluorescamine and detected with a fluorescence detector. The two‐dimensional separation system proved to be reproducible enough to identify some species differences in the tryptic digest fingerprints. Improvements in instrumentation and daily procedures yielding faster analysis times and better reproducibility compared to a previous system are described. Improvements in software yielding gray‐scale images of the two‐dimensional data are described. Identification of selected tryptic fragments was achieved.
No takes yet. Share an insight, caveat, or question.
Bushey et al. (1990) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: