Structure of a polysaccharide protein complex.

Interpretation of the cytochrome c data based solely on a polypeptide or simple protein model leads to an apparent contradiction which is resolved by consideration of coupling of heme and protein transitions.The reciprocal relations required for this coupling are noted and the expected thermodynamic relations are found.Thus, evidence has been presented for the coupling of protein and heme transitions in cytochrome c.The author expresses appreciation particularly to P. Doty, in whose laboratory this work was done, for helpful discussions and for his review of and improvements in the manuscript.