An improved method for quantitating microgram amounts of glycosaminoglycans was applied to the estimation of specific activities of glycosaminoglycans secreted by fibroblastic cells in culture.Following feeding, confluent cultures with serum-containing medium, the glycosaminoglycans secreted into the medium, trypsin-solubilized material fi-om the attached cells,
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Hronowski et al. (1980) studied this question.
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