The behaviour of glutathione in yeast

GLUTATHIONE is found in the cell largely in the reduced condition: yet know- ledge of what reduces it remains much as it was when Hopkins [1921] showed that the disulphide form of glutathione was reduced to the sulphydryl form when it was incubated with kidney, liver, or skeletal muscle. This reduction is due, at least in part, to the presence of a fixed non-autoxidisable -SH group in the protein [Hopkins and Dixon, 1922] which, in reducing soluble disulphide glutathione to the sulphydryl form, becomes itself oxidised to disulphide. Bernheim and Dixon [1928] report that washed liver containing very little fixed -SH reduces much oxidised glutathione.