What question did this study set out to answer?

To develop a rapid, simple, and inexpensive in vitro screening method for type I allergy based on 3H-histamine incorporation and release in human leukocytes.

August 1, 1979Allergy

3H‐Histamine Release from Human Leukocytes

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Authors

PSPer Stahl SkovUniversity of Southern Denmark SNS. NornStatens Serum Institut BWB. WeekeTriumph Adler (Germany)

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Implication

Comparative assay evaluation demonstrates equivalent histamine release between a novel tritiated histamine test and conventional methods in asthmatic patients, indicating feasibility for...

Key Points

To develop a rapid, simple, and inexpensive in vitro screening method for type I allergy based on 3H-histamine incorporation and release in human leukocytes.
Incorporated 3H-histamine into leukocytes in vitro and measured radioactive histamine release following cell provocation with suspected allergens.
Evaluated the method alongside the conventional basophil histamine release technique using cells from 6 asthmatic patients challenged with animal dander, house dust, and mite allergens.
The 3H-histamine release assay demonstrated nearly identical histamine release levels compared with the conventional basophil histamine release method upon allergen challenge.

Cite This Study

Skov et al. (1979) studied this question.

synapsesocial.com/papers/6a8290ad5bcab2ccdb8d5d9e https://doi.org/10.1111/j.1398-9995.1979.tb01707.x

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Implication

Comparative assay evaluation demonstrates equivalent histamine release between a novel tritiated histamine test and conventional methods in asthmatic patients, indicating feasibility for...

3H‐Histamine Release from Human Leukocytes

Discussion

Member takes

Implication

Key Points

Cite This Study

3H‐Histamine Release from Human Leukocytes

Discussion

Member takes

Implication

Key Points

Cite This Study

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