What question did this study set out to answer?

To determine whether caspase-like proteases are activated and functionally required for executing programmed cell death during the pathogen-induced hypersensitive response in plants.

December 5, 2003The Plant CellOpen Access

A Plant Caspase-Like Protease Activated during the Hypersensitive Response

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Authors

NCNina V. ChichkovaLomonosov Moscow State University SKSang Hyon KimMyongji University ETElena S. TitovaLomonosov Moscow State University

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Implication

Biochemical study demonstrates activation of a caspase-like protease during virus-triggered cell death in tobacco plants, indicating its essential role in plant programmed cell death.

Key Points

To determine whether caspase-like proteases are activated and functionally required for executing programmed cell death during the pathogen-induced hypersensitive response in plants.
Inoculated tobacco plants carrying the N resistance gene with Tobacco mosaic virus to trigger the hypersensitive response.
Employed the Agrobacterium tumefaciens VirD2 protein, which contains human caspase-3 cleavage motifs (TATD and GEQD), as a reporter substrate to identify and partially purify plant caspase activity.
Synthesized a cleavage-site-mimicking tetrapeptide aldehyde inhibitor to evaluate its effect on substrate fragmentation in vitro and hypersensitive response execution in vivo.
Ectopically expressed VirD2 reporter proteins underwent specific proteolytic cleavage at the TATD motif during early stages of the Tobacco mosaic virus-induced hypersensitive response.
Proteolytic activity specifically cleaving the TATD target site was partially purified from infected tobacco leaf tissue.
The synthetic tetrapeptide aldehyde inhibitor blocked substrate cleavage by plant and human caspases in vitro and counteracted the execution of the hypersensitive response in vivo.

Cite This Study

Chichkova et al. (2003) studied this question.

synapsesocial.com/papers/6a8293e8d15458f571d10c64 https://doi.org/10.1105/tpc.017889

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Implication

Biochemical study demonstrates activation of a caspase-like protease during virus-triggered cell death in tobacco plants, indicating its essential role in plant programmed cell death.

A Plant Caspase-Like Protease Activated during the Hypersensitive Response

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Implication

Key Points

Cite This Study

A Plant Caspase-Like Protease Activated during the Hypersensitive Response

Discussion

Member takes

Implication

Key Points

Cite This Study

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