What question did this study set out to answer?

To evaluate the abundance, genomic distribution, and practical utility of microsatellite repeats as PCR-based genetic markers in higher plants.

January 1, 1993The Plant JournalOpen Access

PCR‐amplified microsatellites as markers in plant genetics

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Authors

MMMichele MorganteUniversity of Pisa AOAngelo M. OlivieriUniversity of Udine

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Implication

Genomic survey reveals abundant, highly polymorphic microsatellites across higher plant species, highlighting their utility as co-dominant markers for genetic mapping and varietal identification.

Key Points

To evaluate the abundance, genomic distribution, and practical utility of microsatellite repeats as PCR-based genetic markers in higher plants.
Surveyed published plant DNA sequence databases for dinucleotide (≥10 repeats) and trinucleotide (≥7 repeats) motifs across higher plant taxa.
Performed polymerase chain reaction amplification of (AT)n and (TAT)n repeat loci in soybean (Glycine max) to assess polymorphism, somatic stability, and inheritance patterns.
Microsatellite repeats occurred at an average frequency of one every 50 kb across 34 plant species, with AT dinucleotides and TAT trinucleotides representing the most abundant repeat classes.
PCR amplification in soybean demonstrated that microsatellite loci are highly polymorphic due to length variation, remain somatically stable, and segregate in a co-dominant Mendelian pattern.

Cite This Study

Morgante et al. (1993) studied this question.

synapsesocial.com/papers/6a82a1d3d5d5076d1fb285c3 https://doi.org/10.1046/j.1365-313x.1993.t01-9-00999.x

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Implication

Genomic survey reveals abundant, highly polymorphic microsatellites across higher plant species, highlighting their utility as co-dominant markers for genetic mapping and varietal identification.

PCR‐amplified microsatellites as markers in plant genetics

Discussion

Member takes

Implication

Key Points

Cite This Study

PCR‐amplified microsatellites as markers in plant genetics

Discussion

Member takes

Implication

Key Points

Cite This Study

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