Adrenaline-induced enhancement of thrombin-stimulated cytoplasmic alkalinization is a secondary effect not essential for synergistic platelet activation.
Questions necessity of alkalinization for adrenaline-thrombin platelet synergy; leaves open relevance beyond animal models.
Gel-filtrated human platelets were stimulated with thrombin in the absence and presence of adrenaline. Adrenaline markedly enhanced the thrombin-induced increase in cytoplasmic pH (pHi) in BCECF-loaded platelets. This rise in pHi was strongly inhibited by the Na+/H+ exchange blocker EIPA. The potentiation by adrenaline of thrombin-induced PLC activation measured as [32P]PA formation and final platelet responses was, however, not blocked by EIPA, even at low concentrations of thrombin. These results indicate that the enhancement by adrenaline of thrombin-induced cytoplasmic alkalinization may be a secondary effect which is not essential for the potentiation by adrenaline of platelet activation by thrombin.
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Steen et al. (1989) studied this question.
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