Distribution of phospholipid biosynthetic enzymes among cell components of rat liver

Phospholipid EnzymeDistribution in Rat Liver 7961 (specific activity, 60 mCi/mmol) and L-[3-'%]serine (specific activity, 50 mCi/mmol)were products of the Isotope and Nuclear Division of International Chemical and Nuclear Corp., Cleveland, OH. myo-[2-'%]Inositol (specific activity, 11.8 mCi/mmol) was obtained from Calbiochem, La Jolla, CA.Synthetic CDP-dipalmitin was a product of Serdary Research Laboratories, London, Ontario, Canada.Phosphatidic acid, glycerol 3-phosphate, coenzyme A (lithium salt), ATP (Tris salt), CTP (sodium salt), and N-acetylglucosamine were purchased from Sigma Chemical Co., St. Louis, MO.The 250-p HR plates were products of Analabs Inc., North Haven, CT.The phospholipid standards were provided by Prof.