Kineothrix sedimenti sp. nov., a 3-hydroxybutyrate-producing bacterium isolated from sediment of the meromictic Lake Pavin

This is the authors' abstract. We don't add key points for this paper.

An anaerobic, spore-forming, 3-hydroxybutyrate (3-HB)-producing bacterium, strain IPX_CK T , was isolated from sediment of a meromictic lake located in Massif Central (France). Cells were rods, forming filamentous chains which were observed moving under the microscope. Strain IPX_CK T utilized a wide variety of carbohydrates, but not raffinose, rhamnose and starch. Hydrogen (H 2 ), 3-HB, acetate and ethanol were the main fermentative end-products from growth in medium containing glucose. Strain IPX_CK T grew optimally at 37 °C and pH 7. Its closest phylogenetic relative was Kineothrix alysoides (16S rRNA gene sequence identity 98.7%, is DDH 34.6%, ANIb 87.4%). The genomic DNA G+C content was 43.0 mol%. As for K. alysoides , whole-genome sequencing suggested that strain IPX_CK T is capable of fixing nitrogen (N 2 ). However, strain IPX_CK T carried a five- nif -gene-set ( nifHDKEB ), not present in K. alysoides . Genome sequence also showed a high number of encoded chemotaxis receptors (42 genes, the second highest in the family Lachnospiraceae after K. alysoides ). Based on phenotypic, genomic, phylogenetic and chemotaxonomic analyses, it is proposed that a novel species, Kineothrix sedimenti sp. nov., be created, with strain IPX_CK T (DSM 118044 T , CIP 112511 T ) as the type strain.