Transforming Growth Factor-beta 1 Gene Polymorphisms According to Diabetic Nephropathy in Type 2 Diabetes

Background: Transforming growth factor-β is known to play a role in the interaction between metabolic and hemodynamic factors in mediating accumulation of extracellular matrix in the diabetic nephropathy.TGF-β1 gene polymorphism was associated with circulating TGF-β levels and influenced the pathogenesis of fibrotic diseases including diabetic nephropathy.In this study, we examined the relationship between TGF-β1 gene codon 10 polymorphism and type 2 diabetic nephropathy with more than 10-year history of disease. Methods:We conducted a case-control study, which enrolled 325 type 2 diabetes.A total of 176 patients with diabetic nephropathy were compared with 149 patients without diabetic nephropathy.TGF-β1 codon 10 genotyping was determined using polymerase chain reaction with sequence specific primers method.Results: Distribution of TGF-β1 codon 10 genotype in the patients either with nephropathy or without nephropathy is confined to Hardy-Weinberg equilibrium.The patients with nephropathy have higher frequency of TGF-β1 GA/GG genotypes than the patients without nephropathy [GA/GG:AA = 119 (67.6%) : 57 (32.4%) vs. 80 (53.7%) : 69 (46.3%),P < 0.05].Among patients with diabetic nephropathy, those with TGF-β1 GA/GG genotypes had higher serum levels of total cholesterol and LDL-cholesterol. Conclusion:Our results suggest that TGF-β1 gene codon 10 polymorphism may contribute to the type 2 diabetic nephropathy.(J Kor Diabetes Assoc 31:144~150, 2007)