Oligonucleotides from Yeast Tyrosine Transfer Ribonucleic Acid

The separation, base composition, and determination of the nucleotide sequences of the products of pancreatic ribonuclease and RNase T1 digestion of yeast tyrosine transfer ribonucleic acid are described. Analyses of the two digests are in excellent agreement and indicate that the tyrosine RNA is composed of 78 nucleotides. There are 16 residues of 9 different modified nucleotides present. The 5'-phosphate terminal residue is pCp, the first time any nucleotide other than pGp has been detected in this position in a purified transfer RNA.