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September 1, 1989Microbiology

Polymorphic Repetitive DNA Sequences in Mycobacterium tuberculosis Detected with a Gene Probe from a Mycobacterium fortuitum Plasmid

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Authors

ZZZafarina ZainuddinUniversiti Sains MalaysiaJDJeremy W. DaleUniversity of Surrey

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Overview

Laboratory study demonstrates polymorphic repetitive DNA sequences in Mycobacterium tuberculosis isolates, highlighting novel tools for strain fingerprinting and species differentiation.

Key Points

  • To identify and characterize polymorphic repetitive DNA sequences in Mycobacterium tuberculosis using a plasmid-derived probe from Mycobacterium fortuitum.
  • Screened clinical isolates of Mycobacterium tuberculosis via Southern blotting using a probe derived from a Mycobacterium fortuitum plasmid.
  • Isolated two hybridizing M. tuberculosis DNA fragments from a genomic library to serve as probes for restriction fragment length polymorphism (RFLP) profiling.
  • Identified repetitive DNA sequences localized at different chromosomal loci across M. tuberculosis strains, suggesting the presence of a mobile transposable element homologous to the M. fortuitum plasmid.
  • Demonstrated that the resulting probes generate distinct DNA fingerprints among M. tuberculosis isolates and differentiate M. tuberculosis from Mycobacterium bovis and BCG.

Cite This Study

Zainuddin et al. (1989) studied this question.

synapsesocial.com/papers/6a82cbe64bc907ccd675a7eehttps://doi.org/10.1099/00221287-135-9-2347
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Analysis of plasmids in Mycobacterium avium-intracellulare isolates from persons with acquired immunodeficiency syndrome.1986 · 59 citations
  2. 2Lysogeny and transformation in mycobacteria: stable expression of foreign genes.1988 · 329 citations
  3. 3DNA restriction endonuclease analysis of Mycobacterium bovis and other members of the tuberculosis complex1985 · 94 citations
  4. 4Isolation of plasmids from mycobacteria1979 · 60 citations