What Are THE Practical Limits for Detection of Minor Nucleoside Reaction Products with HPLC (UV Detection), H NMR, and TLC (UV Detection)?

Detection limits for the minor component in binary mixtures of Ado/AraA, Ado/XyloA, and Urd/dUrd depend strongly on the combined concentration of analytes. Limiting concentrations (in which ≤1% of the minor component was detected) were about two orders of magnitude lower with HPLC (UV detection) than with 1H NMR and TLC (UV detection) with these nucleosides (εmax 10 000–15 000). Minimum molar percentages of minor components detected in the 0.1–10 mM range were 0.25–1% with HPLC (UV), 1–2% with 1H NMR, and ∼2% with TLC (UV).