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Enzymes have been used in supercritical carbon dioxide (scCO2) rather than in the conventional media to make enzyme reactions greener. This review introduces some enzymatic asymmetric synthesis in scCO2 such as esterification by a lipase and reduction by an alcohol dehydrogenase. A carboxylation by a decarboxylase is also described.
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Matsuda et al. (2004) studied this question.
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