A method is described for measurement of glucose-6-phosphate in tissue extracts by use of glucose-6-phosphate dehydrogenase. Interference resulting from contamination of this enzyme with glutathione reductase was abolished by addition of a mixture of HgCl2 and glutathione. Hexokinase, another contaminating enzyme which interfered with the determination, was inhibited by removing Mg ion. Phosphoglucomutase and phosphoglucoseisomerase were not present in significant amounts.
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Narahara et al. (1960) studied this question.