Trimethylamine biosensor based on pentacene enzymatic organic field effect transistor

This paper reports on an enzymatic organic field effect transistor based biosensor dedicated to the detection of trimethylamine, in view of fish freshness assessment. Flexible devices were processed on Kapton substrate with pentacene as organic semiconductor and Parylene-C as top gate dielectric. Proton sensitive dielectric surface was achieved by depositing a thin hydrogenated silicon nitride layer at moderate temperature on Parylene. An enzymatic membrane made of flavin-containing mono-oxygenase 3 cross-linked with bovine serum albumin deposited on silicon nitride completed the realization of the device. High sensitivity trimethylamine biosensing was demonstrated in the 0–8 ppm range.