Key result
The NET gene G1287A polymorphism was not associated with diabetes, but the AA genotype was more frequent in hypertensive compared to normotensive diabetic patients (19% vs 10%, p<0.05).
Why the study?
Is the G1287A polymorphism in the NET gene associated with type 2 diabetes or hypertension in diabetic patients?
Case-Control (n=670)
Is the G1287A polymorphism in the NET gene associated with type 2 diabetes or hypertension in diabetic patients?
p-value: p=0.43
The G1287A polymorphism in the NET gene is not associated with type 2 diabetes but may increase susceptibility to hypertension in these patients.
AA genotype may link to hypertension risk in diabetes; hypothesis-generating, needs replication before any clinical use.
BACKGROUND: Norepinephrine transporter (NET) is involved in the regulation of norepinephrine (NE) turnover and metabolism. Neuronal NE reuptake may be impaired in individuals with renal disease and/or hypertension due to dysfunction of the NE transporter. A silent G1287A nucleotide substitution in exon 9 of the NET gene was studied in human conditions involving hypertension. We investigated its effect in patients with type 2 diabetes. METHODS: The study involved 215 type 2 diabetes patients with nephropathy, 95 patients with diabetes duration > or =10 years, free of nephropathy, and 360 healthy subjects. All individuals were genotyped for the NET-8 gene polymorphism with the PCR-RFLP method. Genotype and allele frequencies were compared between the groups. NE was measured by high-performance liquid chromatography and electrochemical detection. RESULTS: We genotyped 310 patients and 360 controls for the NET gene polymorphism. Genotype distribution in both groups was in accordance with the Hardy-Weinberg equilibrium. There were no significant differences in the frequency of genotypes and alleles between patients and controls (p = 0.43). The frequencies were also similar for patients with nephropathy and those without. After dividing the patient group into hypertensive (n = 208) and normotensive (n = 102) subjects, there was a significant increase in the frequency of the AA genotype in patients with hypertension compared to normotensives (19 vs. 10%, p < 0.05). CONCLUSION: No association was found between G1287A polymorphism in the NET gene and diabetes. Our results suggest that this polymorphism has a possible role in increased susceptibility to hypertension in patients with type 2 diabetes.
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Książek et al. (2006) conducted a case-control in Type 2 diabetes (n=670). G1287A polymorphism in the NET gene vs. Healthy controls and normotensive patients was evaluated on Frequency of genotypes and alleles between type 2 diabetes patients and healthy controls (p=0.43). The NET gene G1287A polymorphism was not associated with diabetes, but the AA genotype was more frequent in hypertensive compared to normotensive diabetic patients (19% vs 10%, p<0.05).
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