F. M. Areiasa, P. Valentãoa, P. B. Andradea, M. M. Moreiraa, J. Amarala & R. M. Seabrab* a Universidade do Porto , CEQUP/Lab. de Farmacognosia, Fac. Farmácia, R. Aníbal Cunha, Porto, 4050, Portugal b Universidade do Porto , CEQUP/Lab. de Farmacognosia, Fac. Farmácia, R. Aníbal Cunha, Porto, 4050, Portugal * A reversed phase HPLC procedure is proposed for the determination of eight phenolic compounds (2-O-glucosilcoumaric acid, o-coumaric acid, rosmarinic acid, apigenin-7-O-glucoside, coumarin, herniarin, luteolin, and apigenin) in lavender. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS 2 (5 μm particle size: 25.0 × 0.46 cm) column. From the several extractive solvents assayed, only ethanol was able to extract all the mentioned compounds. Best resolution was obtained using a gradient of water-formic acid (19:1) and acetonitrile. Ten samples were subjected to quantification, all of them showing a common composition pattern.
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Areias et al. (2000) studied this question.
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