Key result
The recombinant scFv/6E6 protein against the PEDV S protein was successfully expressed in E. coli and purified using a gel-cutting method followed by electroelution and dialysis.
The successful expression and purification of the recombinant scFv/6E6 protein provides a basis for further antiviral drug research against PEDV.
The variable regions of the heavy chain (VH) and light chain (VL) were amplified by RT-PCR from the hybridoma 6E6, which secretes the monoclonal antibody against PEDV S protein. The VL and VH amplicons were combined using SOE-PCR by a 12 amino acid flexible linker (SSGGGGSGGGGS), which produced the scFv gene (named scFv/6E6). After sequence analysis, the scFv/6E6 gene was cloned into the prokaryotic expression vector pGEX-6p-1 with a GST-tag. The recombinant scFv/6E6 protein was successfully expressed in recombinant Escherichia coli by IPTG induction. Moreover, the recombinant scFv/6E6 protein was purified from the inclusion body form by the gel-cutting measure followed by electroelution and dialysis. The recombinant scFv/6E6 protein reported here will provide some basis for further antiviral drug research based on the scFv molecule.
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Zhu et al. (2013) studied Porcine epidemic diarrhea virus (PEDV). scFv/6E6 recombinant protein was evaluated on Expression and purification of scFv/6E6 protein. The recombinant scFv/6E6 protein against the PEDV S protein was successfully expressed in E. coli and purified using a gel-cutting method followed by electroelution and dialysis.
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