Key result
A dominant-negative mutant of eIF4A more strongly inhibited the in vitro translation of mRNAs containing stable secondary structure in the 5' untranslated region.
Population
in vitro translation system with mRNAs possessing various degrees of secondary structure
Comparison
dominant-negative mutant of mammalian eIF4A vs wild-type eIF4A
Design
Preclinical
Authors
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In vitro findings link eIF4A to structured mRNA translation; leaves open relevance to cardiac gene expression or targeting.
The requirement for eIF4A in translation initiation is directly proportional to the degree of mRNA 5' secondary structure.
Svitkin et al. (2001) studied this question. Dominant-negative mutant of mammalian eIF4A (PRRVAA) vs. Wild-type eIF4A or no added eIF4A was evaluated on Inhibition of in vitro translation of mRNAs with various degrees of secondary structure. A dominant-negative mutant of eIF4A more strongly inhibited the in vitro translation of mRNAs containing stable secondary structure in the 5' untranslated region.
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